To test if the hyperactive egg laying of
nca-1(gf) mutants represented HSN-specific suppression of Go(Q205L) or a bypass, we created
nca-1(gf) mutant animals lacking HSNs using the
egl-1(
n986dm) mutation that causes premature death of the HSN neurons (Conradt & Horvitz,1998). Unlike the results with Go(Q205L),
nca-1(gf) only partially suppressed the egg-laying defects caused by loss of HSNs, suggesting NCA-1 NALCN channels promote egg-laying behavior both in and outside of the HSNs.