Addition of the RIM C2A domain to the PDZ domain of RIM (GFP-PDZC2ARIM), but not the C2A domain of RIM alone (GFP-C2ARIM), was sufficient to localize RIM and restore ELKS localization. Interestingly, the localization of GFP-PDZC2ARIM is dependent on ELKS expression.
"UNC-13L C2A domain tightly binds the UNC-10/RIM ZF domain, and that this interaction was eliminated by a double mutation in the ZF domain (K77/79E), which is analogous to mutations that disrupt binding of the corresponding mammalian Munc13-1 and RIM domains"
"UNC-13L C2A domain tightly binds the UNC-10/RIM ZF domain, and that this interaction was eliminated by a double mutation in the ZF domain (K77/79E), which is analogous to mutations that disrupt binding of the corresponding mammalian Munc13-1 and RIM domains"
"Caenorhabditis elegans ELKS is an active zone protein that directly interacts with the postsynaptic density-25/Discs large/zona occludens (PDZ) domain of RIM."
Antibody specific to Nup35 gave rise to clear nuclear rim staining in control embryos, Nup35 was completely absent from the chromatin of npp-8 RNAi embryos.
In unc-104(e1265) animals, although synaptic vesicle proteins and synaptic vesicles accumulate in neuronal cell bodies11, Rim was still concentrated in puncta in the nerve cord.
"AMPH-1 co-immunoprecipitated the mini-ANC-1 protein and vice versa (Figure 3A). To confirm this biochemical interaction, we examined whether ANC-1 and AMPH-1 co-localize. Endogenous ANC-1 accumulated at the nuclear rim and also decorated perinuclear structures (Figure 3B-3D). Similarly, AMPH-1 was present around the intestinal nuclei and partially co-localized with ANC-1 (Figures 3B-3D and S3A-S3D)"
"AMPH-1 co-immunoprecipitated the mini-ANC-1 protein and vice versa (Figure 3A). To confirm this biochemical interaction, we examined whether ANC-1 and AMPH-1 co-localize. Endogenous ANC-1 accumulated at the nuclear rim and also decorated perinuclear structures (Figure 3B-3D). Similarly, AMPH-1 was present around the intestinal nuclei and partially co-localized with ANC-1 (Figures 3B-3D and S3A-S3D)"