daf-12A expression was analyzed with
ife-4(RNAi) in worms expressing an integrated
daf-12A::GFP array. Animals were scored for fluorescence at the L2/L3 developmental stage. Many neurons and neuronal cell bodies around the pharynx, the VNC, and hypodermal cells were fluorescent in controls, as described previously. However,
ife-4(RNAi) feeding reduced DAF12A::GFP fluorescence in pharyngeal neurons, especially in neuronal cell bodies, and in the hypodermis.
ife-4(RNAi) had no effect on GFP expressed from other promoters, such as those corresponding to a heat shock protein (
hsp-16.2::GFP) or to a touch receptor neuron protein (
mec-3::GFP).