Our analysis of the region around
unc-15(I) has led to the construction of the following complementation map. In Waterston, et al.'s paper (1977), they reported that
e73,
e1215, and
e1402 are complementing alleles of
unc-15. These alleles all fail to complement
e1214, a mutation that prevents the production of paramyosin. We have extended the complementation analysis to include three additional unc- 15 alleles:
su228 and
su2000 (from Henry Epstein and Janice Zengal) and
st8 (from Bob Waterston). To our knowledge these are the only alleles of
unc-15 in existence, but we would happily stand corrected on this fact. We would like to map any other alleles that exist. The diagram below illustrates the pattern of complementation for all but
e1402 which is temperature sensitive (analysis in progress). All alleles fail to complement
e1214 which 18 nullo for paramyosln. Failure to complement is indicated in the figure by overlapping lines. [See Figure 1] It is premature to speculate about the meaning of the complementation pattern for
unc-15 alleles. However we hope to eventually understand the pattern by comparing the complementation data to genetic fine structure analysis of
unc-15, recombinant DNA analysis of genomic clones for the
unc-15 region, and protein chemistry of the paramyosin molecule. Currently, Peter Candido and we are analyzing cyanogen bromide fragments of wild type and mutant paramyosins. We hope by this analysis to correlate a portion of the intragenic recombination map with the peptide map. We are interested in understanding the organization of the
unc-15 gene and the genes immediately around
unc-15. As part of this analysis, I have constructed a partial fine structure map of
unc-13. Two of the alleles mapped are suppressed by
sup-5 and we take this as evidence that these alleles,
e450 and
e1091 map into the coding portion of
unc-13. This map and the
unc-15 map are illustrated below. The map is based on intragenic fine structure data (published in the November, 1980 Issue of Genetics), and two- and three-factor mapping data for
e73 and
e51. We are currently screening our genomic library ( constructed by Susan Bektesh, Seattle) for clone(s) of the
unc-15 Eventually we hope to compare our genetic map with a recombinant DNA analysis of this region of the genome. [See Figure 2]