Microtubules are critical to a number of vital cellular processes, including cell division, intracellular transport, cell movement, and cell structure. Microtubule-associated proteins (MAPs) facilitate these various microtubule functions by regulating dynamic instability, crosslinking, and trafficking, among others (Alfaro-Aco and Petry 2015). There are over 60 conserved MAPs in Caenorhabditis elegans, many with essential functions (Oegema 2006; Lacroix et al. 2014; Rose and Gonczy 2014; Quintin et al. 2016). Not surprisingly, microtubules and MAPs are required during early Caenorhabditis elegans embryogenesis when cell divisions predominate (Oegema 2006; Rose and Gonczy 2014). Temporally-controlled microtubule disruption by either drugs or transgenic constructs can bypass these early requirements and have shown that microtubules are also necessary for proper epidermal morphogenesis during mid-embryonic development (Williams-Masson et al. 1998; Quintin et al. 2016). However, it is still unclear what complement of MAPs function during this stage of development to pattern the overall microtubule network and control dynamic instability.C. elegans UNC-33 is an ortholog to collapsin response mediator proteins (CRMPs), a family thought to regulate the dynamic instability of microtubules (Li et al. 1992; Fukata et al. 2002; Tsuboi et al. 2005; Lin et al. 2011). Mutants of
unc-33 exhibit defects in axonal outgrowth and guidance, resulting in paralysis and locomotor defects (Hedgecock et al. 1985; Li et al. 1992). The dendrites of sensory neurons in these animals have far more microtubules than in wildtype and they are often larger in diameter with other structural defects (Hedgecock et al. 1985). In addition to these neuronal effects, homozygous mutant animals of the classic allele,
unc-33(
e204), also appear shorter and stouter than wildtype animals. While some Dumpy animals are caused by mutations in genes important for cuticle development (Kusch and Edgar 1986), others are caused by mutations in genes important for embryonic epidermal morphogenesis, such as
sma-1 (McKeown et al. 1998; Praitis et al. 2005),
let-502 (Piekny et al. 2000; Gally et al. 2009; Quintin et al. 2016), and
lin-26 (Ferguson and Horvitz 1985). Two other C. elegans gene products, DHP-1 and DHP-2, are almost 70% identical in sequence with UNC-33 and are also evolutionarily related to vertebrate CRMPs (Takemoto et al. 2000). In situ hybridization showed
dhp-1 mRNA in hypodermal cells from late gastrula to at least 2-fold body elongation, and a GFP-tagged version of DHP-1 was also expressed in the larval hypodermis (Takemoto et al. 2000). There are currently no associated phenotypes to
dhp-1, so we hypothesized that UNC-33 and DHP-1 may be functionally redundant in the epidermis during C. elegans embryogenesis.To determine if any functional redundancy exists between UNC-33 and DHP-1, we used
unc-33(
e204) homozygous animals as a sensitized background and disrupted
dhp-1 expression by feeding RNA interference (RNAi). Because we were specifically interested in potential embryonic effects, we scored the number of embryos that failed to hatch and took this as a percentage of the total progeny (Figure 1). When analyzed by two-way ANOVA, a significant interaction was found between the strains and gene knockdowns (p=2.761e-07). We used post-hoc Tukeys honest significant test to make all pairwise comparisons; this revealed which of the gene knockdowns interacted with
unc-33(
e204) mutants. As expected for a negative control, the empty feeding vector (L4440) fed to either wildtype or
unc-33(
e204) homozygous animals showed a low level of lethality, 3.4% and 9.3% on average, respectively. These data were not significantly different from each other in a pairwise comparison (p=0.872). In wildtype animals, the positive control,
hmp-2(RNAi), resulted in 17.9% of embryos that failed to hatch but this was not found to be significantly different to the L4440 control (p=0.068). It is important to note, however, that less than 1% of hatched
hmp-2(RNAi) animals were normal larva; the vast majority were Hmp with severe body morphology defects. Surprisingly,
unc-33(
e204);
hmp-2(RNAi) resulted in 65.3% of embryos that failed to hatch, a significant increase compared to both the negative control in the same strain and
hmp-2(RNAi) in wildtype (p < 0.001 for both). In both wildtype and
unc-33(
e204) homozygous animals, knockdown of
dhp-1 did not significantly alter the percent of embryos that failed to hatch (3.1% and 19.5%, respectively).