Identified through forward genetics,
spe-9 was the first gene to be identified in C.&#
xa0;elegans as necessary for fertilization.<sup>1</sup> Since then, genetic screens in C.&#
xa0;elegans have led to the identification of nine additional sperm genes necessary for fertilization (including
spe-51 reported by Mei et&#
xa0;al.<sup>2</sup> and the
spe-36 gene reported here).<sup>3</sup><sup>,</sup><sup>4</sup><sup>,</sup><sup>5</sup><sup>,</sup><sup>6</sup><sup>,</sup><sup>7</sup><sup>,</sup><sup>8</sup><sup>,</sup><sup>9</sup> This includes
spe-45, which encodes an immunoglobulin-containing protein similar to the mammalian protein IZUMO1, and
spe-42 and
spe-49, which are homologous to vertebrate DCST2 and DCST1, respectively.<sup>4</sup><sup>,</sup><sup>7</sup><sup>,</sup><sup>8</sup><sup>,</sup><sup>10</sup><sup>,</sup><sup>11</sup><sup>,</sup><sup>12</sup><sup>,</sup><sup>13</sup> Mutations in any one of these genes result in healthy adult animals that are sterile. Sperm from these mutants have normal morphology, migrate to and maintain their position at the site of fertilization in the reproductive tract, and make contact with eggs but fail to fertilize the eggs. This same phenotype is observed in mammals lacking Izumo1, Spaca6, Tmem95, Sof1, FIMP, or Dcst1 and Dcst2.<sup>10</sup><sup>,</sup><sup>14</sup><sup>,</sup><sup>15</sup><sup>,</sup><sup>16</sup><sup>,</sup><sup>17</sup><sup>,</sup><sup>18</sup><sup>,</sup><sup>19</sup> Here we report the discovery of SPE-36 as a sperm-derived secreted protein that is necessary for fertilization. Mutations in the Caenorhabditis elegans
spe-36 gene result in a sperm-specific fertilization defect. Sperm from
spe-36 mutants look phenotypically normal, are motile, and can migrate to the site of fertilization. However, sperm that do not produce SPE-36 protein cannot fertilize. Surprisingly,
spe-36 encodes a secreted EGF-motif-containing protein that functions cell autonomously. The genetic requirement for secreted sperm-derived proteins for fertilization sheds new light on the complex nature of fertilization and represents a paradigm-shifting discovery in the molecular understanding of fertilization.