We have isolated a set of eight dominant, temperature-sensitive, maternal-effect lethal mutations which define at least five independent loci. As reported in the last WORM BREEDER'S GAZETTE,
ct46/ct46(I) and
ct46/+(I) animals produce embryos with early cleavage defects reminiscent of those resulting from defects in the
zyg-9(II) gene (1), a maternal effect locus defined by six recessive mutations ( 2). Mutations at both loci result in a failure of pronuclear fusion, a longitudinal first cleavage, and subsequent multiple nuclei per cell. If these two loci are interacting to control the same process, a synergistic effect might be found when the two mutations are present in the same individual. We have constructed animals carrying both
ct46 and the temperature sensitive allele
zyg-9(
b244) and/or the putative null allele
zyg-9(
b301), which is an amber mutation. At 20 , the embryos produced by
ct46/+ animals show an intermediate level of viability (33% hatch, see Table 1). At this temperature the embryos produced by
b244 animals are essentially fully viable (though probably near some threshold, since viability drops to zero at 25 ). However, the embryos produced by
ct46/+;
b244/b244 animals at 20 show an 800- fold lower level of viability than predicted if the effects of the two mutations were independent. Little or no interaction was seen in
ct46/+;
b244/+. Lowering the level of the
zyg-9 gene product by half with the null allele (
ct46/+;
b301/+) also has little or no effect. The combination of
b244 and
b301 (
ct46/+;
b244/b301) does show the synergistic effect. Results obtained with a few other mutant combinations are shown in the table. These results show that
ct46 acts as a dominant enhancer of zyg- 9
(b244) (or
zyg-9(
b244) acts as a recessive enhancer of
ct46). This effect, together with the similarity of phenotypes resulting from the
ct46 and
zyg-9 mutations, is circumstantial evidence for a specific interaction between these loci. As a more direct test of such an interaction, we will try to isolate extragenic suppressors of
ct46 and see if any are found in zyg- 9, and vice versa. We are also testing other alleles of
zyg-9 for interactions with
ct46.