Supplemental Figure S3.
acr-15 and
acr-16 expression patterns. (A) Expression pattern of
acr-15 determined by a transgene expressing YFP under the
acr-15 promoter (~1.6kb). Shown in the left panel is a YFP fluorescence image. Cells expressing YFP are listed in the right panel. (B) Expression pattern of
acr-16 determined by a transgene expressing YFP under the
acr-16 promoter (~6kb). Shown in the left panel is a YFP fluorescence image. Cells expressing YFP are listed in the right panel.
acr-16 showed stronger expression in muscle cells compared to
acr-15. (C) Chronic nicotine treatment up-regulates ACR-15::YFP expression in the command interneuron AVA. ACR-15::YFP is a functional fusion as it can rescue
acr-15 mutant phenotypes (data not shown). AVA was chosen for analysis because of its relatively easy identification in adult worms, its essential role in nicotine-dependent behavior and its robust calcium responses to nicotine stimulation. Worms were chronically treated with 1.5 uM nicotine for ~16 hours before imaging. (D) Histogram of ACR-15::YFP expression level in AVA in wild-type worms. *p<0.005 (t-test). (E) Histogram of ACR-15::YFP expression level in AVA in
trp-2 mutant worms. *p<0.005 (t-test). The same ACR-15::YFP transgene from (D) was crossed into the
trp-2 mutant background.