Figure S2 Monitoring of granule formation under oxidative stress, heat-shock or aging by using other reporter markers. (A) Representative confocal images of 1-day adults expressing the translational fusion
dcap-1::gfp (BRF155 in Table S1) or
ife-2::gfp (BRF70 in Table S1) under normal conditions (-SA) or after exposure to 10 uM sodium arsenite for 3h(+SA) at 25uC. (B) Representative confocal images of 1-day adults expressing the translational fusion
ain-1::gfp (MH2704 in Table S1), the transcriptional fusion of
ife-2 putative promoter to gfp (BRF68 in Table S1) or the translational fusion
ife-2::gfp (BRF70 in Table S1) under normal conditions (-HS) or after heatshock at 35C for 3 h (+HS). The same transgenic animals are shown at the day 5 of adulthood, grown under normal conditions, at 25C. (C) Representative confocal images of 1-day adults coexpressing
ife-2::gfp and
dcap-1::rfp (BRF313 in Table S1). Arrows point to induced granules. Scale bar: 25 mm. (D) Expression levels of
ain-1 gene in N2, as 1-day or 5-day adults grown under normal conditions (-HS) or as 1-day adults exposed to heat-shock at 35uC for 3 h (+HS), measured by quantitative RT-PCR and normalized to
ama-1(mRNA) levels. Error bars show the SD of the means of two independent experiments.