Figure S4. LIN-17/CAM-1 Heteromers Expressed in Muscle Arms, Related to Figure 3 (A) Images of YFP expression in the tips of muscle arms in transgenic worms that carried either the Pmyo-3::
cam-1(DTKD)::n-yfp transgene (left), the Pmyo-3::lin- 17::c-yfp transgene (middle), or both transgenes (right). Worms that carried both transgenes were cross progeny of worms that carried only one of the two transgenes. Scale bar, 1 mm. (B) Top and lower left panels show images of either single (top) or multiple (lower left) muscle arms in transgenic WT and mutant worms that expressed soluble mCherry under control of the
myo-3 muscle-specific promoter. Scale bars, 1 mm (top); 2 mm (lower left). Quantification of muscle arm density (lower right panel). For WT and
cwn-2 mutants, n = 5. (C) ACR-16::GFP expression in muscle arms of WT,
lin-17(
n671), and
lin-17(
n671) transgenic mutants that expressed a WT
lin-17 transgene in neurons. Scale bars, 1 mm. Quantification of ACR-16::GFP fluorescence intensity relative to WT (n R 10 for all genotypes. *p < 0.05). (D) UNC-29::GFP and UNC-49::GFP expression in muscle arms of transgenic WT and
cwn-2 mutants. Scale bars, 1 um. Error bars represent SEM.