Figure 1 Cl- channel CLH-1 is needed in AMsh glia for HCO3- efflux:(A) Schematic representation of the chloride channel CLH-1 in glia and its function. CLH-1 mediates both efflux (big arrowhead) and to a lesser extent influx (small arrowhead) of Cl- and HCO3- thereby regulating the concentration of Cl- and pH both inside and outside of the cell (Grant et al., 2015, Fernandez-Abascal et al., 2021). (B) Representative images of wild type (N2, left column) and
clh-1(
ok658) mutants (
clh-1, right column) expressing the pH sensor Superecliptic pHluorin (pGM87) under the control of the AMsh glia specific promoter pT02B11.3, grown in normal conditions (NGM, top row) or in NGM plates supplemented with 150 mM KHCO3 (bottom row). Scale bar is 50 µm. (C) Violin plots showing the quantification of relative pH inside the AMsh glia as pixel intensity of pGM87 in wild type (red) and
clh-1(
ok658) mutants (blue) grown on NGM or NGM supplemented with KHCO3 plates. Data were normalized to N2 in NGM conditions. Each data point represents one worm (n= 58, 31, 26 and 74, respectively). Statistics were done by ANOVA followed by Tukey post-test (****p<0.0001).