Northern analysis of C. elegans miR-248 during larval development. Total RNA was analyzed from mixed-stage N2 worms (M), embryos (E), larval stages (L1, L2, L3, L4), adults (A),
glp-4(
bn2) adults (G), N2 dauers (D), mixed-stage
him-8(
e1489) worms (H), and N2 starvation-arrested L1 larvae (sL1). To control for loading, the blot was reprobed for the U6 snRNA (U6). Normalizing to the U6 signal, the miR-248 signal was fourfold greater in dauer than in most other stages, except for
glp-4 adults, in which it was twofold greater.