Figure S4. Mislocalization of CPC components and CPC-related histone marks in
syp-1(12A) mutants. (A) Immunostaining of -2, -1, and prometaphaseoocyte nuclei with SYP-1 (magenta) and ICP-1 (green) antibodies in the
plk-2 (
ok1936) gonad. Although ICP-1 is not detected in 1 oocyte nuclei, accumulation of ICP-1 is found in oocyte prometaphase nuclei. Arrowheads indicate sperm nuclei. (B and C) Maximum-intensity projection images of the whole -1oocyte nucleus with ICP-1 (green, top) or H3T3ph (green, middle) and HTP-3 (magenta) staining in
syp-1(wt) (top),
syp-1(
me17);
syp-1(12A) (middle), andsyp-1
(me17) (B and C, bottom) and
syp-1(T452A);
syp-1(
me17) (B) animals. Partial projections of the chromosomes indicated by circles are presented withthe long axis rotated to the vertical in Fig. 4 (B and C). Both ICP-1 and H3T3ph staining levels are reduced in 12A mutants. Arrowheads indicate spermDNA. (D) Immunostaining of histone H3S10ph (green) and HTP-3 (magenta) in 1 oocyte nuclei of wild-type N2 and
syp-1(12A);
syp-1(
me17) animals.H3S10ph staining levels are reduced in 12A mutants. Bars, 5 um.