Figure 5. MES-6 distribution in adults and embryos. (A) Affinity-purified rabbit anti-MES-6 antibody and preimmune serum from the rabbit were used to detect proteins from a C. elegans embryo homogenate on a western blot. Adult N2 or mes mutant hermaprodites (B-F) and their embryos (G-K) were stained with affinity-purified anti-MES-6 (left) and the DNA dye, DAPI (right). (B,G) MES-6 localizes to germ nuclei, including oocytes, in the gonads of wild-type adult hermaphrodites (B). MES-6 is also faintly detectable in the larger nuclei of the gut (upper nuclei in B). MES-6 is observed in all nuclei in wild-type embryos (G). (C,H) MES-6 staining is greatly reduced or undetectable in the germlines of homozygous
mes-2(
bn11) mutants (C) and in their embryos (H). (D,I) MES-6 staining appears wild type in the germlines of
mes-3(
bn35) homozygotes (D) but is greatly reduced or undetectable in their embryos (I). (E,J) MES-6 staining appears wild type in
mes-4(
bn67) mutant mothers (E) and in their embryos (J). (F,K) MES-6 staining is greatly reduced or undetectable in the germlines of
mes-6(
bn38) homozygotes (F) and in their embryos (K). Scale bars, 50 µm (B-F) and 10 µm (G-K).