Figure 1
rol-9(
sc148) is a gain of function allele of
mlt-11:(A) Fosmid rescue experiments define W01F3.3 as the gene encoding
rol-9. The structure of fosmid W01F3 is shown, with restriction enzyme sites (S: SacI, K: KpnI, A: AatII) and protein-coding genes annotated. Below, representations of restriction fragments are shown with their rates of rescue shown at the right side. Full bars represent the sequence retained in the fragment. (B)
sc148 is a homozygous deletion in
mlt-11. The deduplicated read depth across the
mlt-11 locus is shown as grey bars. A 205 basepair deletion in
mlt-11 is noted. Below, a representation of the gene structure in
mlt-11 is annotated with the positions of functional domains. (C)
sc148 replaces 205bp with GGGG, making an in-frame replacement of 68aa with Arg-Gly. The wildtype and mutant sequences flanking
sc148 are shown. (D)
sc148 deletes a peptide conserved in Rhabditina nematodes. Shown is a multiple sequence alignment of
mlt-11 orthologs in 7 species of nematodes. The positions of an upstream Kunitz inhibitor domain and the sequence deleted in
sc148 is annotated below the alignment. Amino acid positions are numbered based on C. elegans
mlt-11.