Figure 3. Phosphorylation of Ser49 of eIF2a by PEK-1 in the ASI Neuron Pair Promotes Entry into Dauer Diapause (A) Fractions of animals with indicated genotypes entering the dauer stage at 25C. The
daf-28(
sa191) strain in this figure represents nontransgenic controls from all three lines. (B) Fractions of animals with indicated genotypes entering the dauer stage at 25C. The
daf-28(
sa191);
pek-1(
ok275) strain in this figure represents nontransgenic controls from all three lines. p < 0.01 was determined by paired, two-tailed Student's t test (nontransgenic fraction versus transgenic fraction for each line). Results from the ASI-specific
gpa-4 promoter are shown in this figure (referred to as PASI). Mean 6 SD is plotted. The number of trials and animals scored is documented in Table S2. See also Figure S3. (C) Schematic for the mechanism by which ER stress in the ASI neuron pair promotes entry into dauer diapause.