Figure 4. LIN-61 is an ubiquitously expressed nuclear protein with punctate localization. (A) Affinity-purified antibodies raised against recombinant LIN-61 were used to blot extracts from both wild-type and
lin-61(
n3809) mutant animals. Asterisks denote nonspecific immunoreactivity. HM4077 antibodies were raised in a guinea pig. HM4078 antibodies were raised in a rabbit. (B, D, F, H, J, and L) Whole-mount staining with anti-LIN-61 antisera. HM4077 was used for whole-mount staining of embryos, and HM4078 was used for whole-mount staining of adults. (B) LIN-61 is expressed in discrete foci in the nuclei of the developing embryo. (C) 4',6-diamidino-2-phenylindole (DAPI) staining of the embryo shown in (B). (D) Enlargement of the boxed portion of B. (E) Enlargement of the boxed portion of C. (F) LIN-61 was absent in
lin-61(
n3809) embryos. (G) DAPI staining of the embryo shown in F. (H) LIN-61 was broadly expressed in the adult hermaphrodite germline and was localized to condensed chromosomes. (I) DAPI staining of the germline shown in H. (J) Enlargement of the boxed portion of H. (K) Enlargement of the boxed portion of I. (L) LIN-61 staining was absent from the germline of
lin-61(
n3809) adult hermaphrodites. (M) DAPI staining of the germline shown in K. WT, wild type. Bars, 10 μm.