vit-2 functions downstream of
pry-1 to regulate lipid levels and lifespan. (A) qRT-PCR analysis of vit genes in
day-1 and
day-4 pry-1(
mu38) adults. The relative normalized expression has been plotted. Each data point represents the mean of at least two replicates and the error bar represents the SEM. *p<0.05 and **p<0.01, compared to wildtype (N2) which is normalized to one. (B-E) Oil Red O analysis of N2 and
pry-1 mutants following control and
vit-1/2 RNAi treatments. B, D) DIC micrographs of representative
pry-1(
mu38) and N2
day-4 adults. (C, E) Quantification of Oil Red O signal from two replicates. (F, G) Lifespan analysis of
pry-1(
mu38) and N2 animals following adult-specific knockdown of
vit-1/2. Mean lifespan increased by 102% (6.3 +/- 0.6 compared to 3.1 +/- 0.3, p<0.001) for
pry-1(
mu38) and 16.6% (19.6 +/- 0.8 compared to 16.7 +/- 0.8, p<0.01) for N2.