Figure 3
ptr-6 is expressed in and acts in the hypodermis to regulate membrane integrity. (A) Gene structure of
ptr-6. The
ys9 and
ys20 mutation sites are indicated as lines. The red box indicates the region deleted in the
ok2988 allele. (B) Predicted protein structure of PTR-6 and the sites of the
ys9 and
ys20 mutations. The red box is the deleted portion of PTR-6 in the
ok2988 allele. The blue box indicates the putative SSD domain. (C)
ptr-6 expression pattern. A transgenic construct with the
ptr-6 promoter fused to GFP is expressed along the hypodermis. Signal intensity was strong in the head region. Bar (upper), 100 um; bar (lower), 20 um. (D)
ptr-6tissue-specific rescue experiment. A hypodermis-specific
ptr-6 expressionline,
ptr-6(
ys20);[Pdpy-7::
ptr-6::GFP], shows altered ethanol sensitivity similar to wild type. In contrast, a muscle-specific expression line,
ptr-6(
ys20);[Pmyo-3::
ptr6::GFP], did not show any difference compared to the
ptr-6(
ys20) mutant.