(A)
grk-2 is expressed in commandinterneurons. Representative images of a Z-stack projection of the area around the nerve ring (head) of an animal coexpressingtagRFP fused to the GRK-2 cDNA driven by the
grk-2 promoter (
grk-2::RFP, transgene yakIs19) and a
cho-1 fosmid YFP reporter(
cho-1fosmid::SL2::YFP::H2B, transgene otIs534). For the
cho-1 fosmid reporter, an SL2-spliced, nuclear-localized YFP::H2Bsequence was engineered right after the stop codon of the gene [93,110]. As indicated in the figure,
grk-2::RFP is expressed in theAVA, AVB, AVD, and AVE command interneurons; SMD and RMD head motor neurons; and in the AIN, AIY, SIA, SIB, and SAAinterneurons. Scale bar: 10 um.