Figure S5 Bilaterally symmetric expression and function of hlh- 14 in the I1L/I1R lineage. A: Ventral view of the 3D-poitions of the bilaterally symmetric I1L/I1R neuroblasts (light blue) at the AB256-cell stage. The gut lineage is shown for positional information. Cell coloring after [10]. B: Lineage and
hlh-14 expression analysis of the I1L/I1R lineage in wildtype (containing the ntIs1 transgene),
hlh-14(
tm295); ntIs1 and
hlh-14(
tm295); otEx4507, Ex[hlh-14FOS::yfp]; ntIs embryos. Purple lines indicate the expression of
hlh-14 (assessed in
hlh-14(
tm295); Ex[hlh-14FOS::yfp embryos) and the numbers below indicate the number of lineages that divided as indicated and the total number of left or right lineages followed to terminal division are shown at the bottom of each lineage diagram indicate the total number of lineages followed. The mother of the I1L/I1R blastomeres divides early in
hlh-14 mutants, at a time when sister lineage divides to produce one pharyngeal marginal cell (
mc1) and one pharyngeal muscle cell (
m3V). This defect is rescued with the hlh-14FOS::yfp transgene. Red cell names indicate neurons/glia and green cell names indicate mesoderm.