Figure 4. Effects of Cry5B on LEC-8::EGFP expression. Images of representative expression patterns of
lec-8::gfp transgenic animals that were fed E. coli JM103[pQE9] (A-D) or JM103[pQE9(Cry5B)] (E-H) are shown. A, adult showing EGFP fluorescence in pharyngeal-intestinal valve cells (arrowheads) and in the tail region (arrows). B, head regions of an adult showing EGFP fluorescence in pharyngeal-intestinal valve cells. C, EGFP fluorescence in the intestine. D, adult with EGFP expression in a small group of cells around the intestinal-rectal valve. E, wild-type N2 expressing LEC-8::EGFP was fed E. coli JM103[pQE9(Cry5B)]. F and G, adult showing EGFP fluorescence in intestinal nuclei (asterisk) and membrane (stars). H,
bre-5(
ye17) worms fed E. coli JM103[pQE9(Cry5B)]. Exposure times were identical for each pair of wild-type N2 and
bre-5(
ye17) images. Scale bars, 100 μm (A, E, and H) and 20 μm (B-D, F, and G).