Picture from Bellanger JM et al. (2003) Curr Biol "TAC-1 and ZYG-9 form a complex that promotes microtubule assembly in C. elegans ...."

Figure 3. TAC-1 Is Present in the Cytoplasm and at Centrosomes(A-K) One-cell-stage embryos stained with antibodies against TAC-1 and alpha-tubulin. The panels on the left show anti-TAC-1 staining, and the panels on the right show a merge of TAC-1 (red), alpha-tubulin (green), and Hoechst counterstain to view DNA (blue). (A-D) Wild-type. TAC-1 is slightly enriched at spindle poles during the (A) meiotic division, and more so at centrosomes in one-cell-stage embryos during (B) prophase and thereafter (C and D) during the cell cycle. Moreover, TAC-1 is present in the cytoplasm throughout the cell cycle. Note weak enrichment on the spindle during metaphase (C), particularly in the vicinity of the chromosomes (arrow). (E) tac-1(RNAi), prometaphase. Both centrosomal and cytoplamic signals are essentially absent. (F-H) (F) dhc-1(RNAi), mitosis; (G) cold-treated, metaphase; (H) tba-2(RNAi), mitosis. In all three cases, TAC-1 distribution is as in wild-type. (I) spd-5(or213). Although TAC-1 is not enriched in a structure that resembles centrosomes, note the weak enrichment around the chromosomes. (J) tbg-1(t1465), metaphase equivalent. Centrosomal TAC-1 is diminished. (K) air-1(RNAi), anaphase equivalent. The TAC-1 signal at spindle poles is minimal. Note also the slight enrichment between the spindle poles.

Picture from Bellanger JM et al. (2003) Curr Biol "TAC-1 and ZYG-9 form a complex that promotes microtubule assembly in C. elegans ...."

TAC-1 Is Present in the Cytoplasm and at Centrosomes(A-D) One-cell-stage embryos stained with antibodies against TAC-1 and alpha-tubulin. The panels on the left show anti-TAC-1 staining, and the panels on the right show a merge of TAC-1 (red), alpha-tubulin (green), and Hoechst counterstain to view DNA (blue). (A-D) Wild-type. TAC-1 is slightly enriched at spindle poles during the (A) meiotic division, and more so at centrosomes in one-cell-stage embryos during (B) prophase and thereafter (C and D) during the cell cycle. Moreover, TAC-1 is present in the cytoplasm throughout the cell cycle. Note weak enrichment on the spindle during metaphase (C), particularly in the vicinity of the chromosomes (arrow).