Fig 2.
chdp-1 functions cell-autonomously. (A) The C10G11.7 gene (
chdp-1) encodes a single CH domain-containing protein. The molecular lesions in
xd27 and
tm4947 are indicated. P1 and P2: proline-rich regions. Helix: amphipathic helix. (B-C) Quantification of BDU (B) and PLM (C) cell protrusion size using Punc-86::Myr::GFP in wild type,
chdp-1(
xd27),
chdp-1 (
tm4947), and the corresponding
chdp-1 rescuing strains. n=30. (D-E)
chdp-1 gene expression in an embryo (D) and an adult animal (E) revealed by Pchdp-1::GFP. In the adult, the BDU and PLM cell bodies are boxed. (F) Rescue of the BDU-PLM disconnection phenotype by tissue-specific expression of
chdp-1 using different promoters: Punc-53 in BDU; Pmec-7 in PLM; Pchdp-1 in both BDU and PLM cells; Phyp-7 in epidermal cells; Pmyo-3 in muscle cells. n=100. For all quantification analyses, error bars represent the standard error of the mean (SEM); ***p < 0.001; **p < 0.01; *p < 0.05; NS, not significant.