Figure S4: Ortholog of human ENPP1 (C27A7.1) is expressed in the EV-releasing ciliated sensory neurons (IL2s, CEMs, RnBs, and HOB) of C. elegans, related to Figure 4.
x2028;(A) Alignment of the phosphodiesterase domain of C27A7.1 to ENPP1 (nucleotide-specific) and ENPP2 (lysophospholipid-specific hydrolase) of human and mouse suggests that C27A7.1 is a nucleotide-specific hydrolase. Black boxes indicate conserved regions forming substrate-binding pockets from [S2]. Most similarity is observed between C27A7.1 and ENPP1. Figure bracket indicates a linker region of ENPP1 critical for its nucleotide substrate recognition; mutagenic studies show that loss of this linker grants ENPP1 the ability to hydrolase lysophospholipids, yet at much lower efficiency than ENPP2. (B) Overall view of the male head with prominent ENPP-1::mScarlet presence in the pharynx, as well as in CEM and IL2 neurons.
x2028;(C-E) Maximum intensity projections showing presence of ENPP-1 in cilia of RnB neurons (C), IL2 neurons (D), and CEM neurons (E). Ci - cilia.