Figure 5. Ectopic Wnt expression rescued compound Wnt mutants. (A-F) The expression of
cwn-1p::CWN-1::Venus (A),
cwn-2p::CWN-2::Venus (B, C),
ceh-22p::CWN-1::Venus (D), and
ceh-22p::CWN-2::Venus (E). Anterior is to the left and ventral to the bottom, except for (B), in which anterior is to the upper-left and ventral toward the right. Scale bars: 20 mm. Confocal images in (A, B) and (D, E), were focused on hypodermal cells. The focus is on the pharynx/intestine in (C). (A, B) Dashed lines outline seam cell nuclei. White arrowheads and blue arrows indicate some of the GFP puncta and granule autofluorescence, respectively, which were observed even in the corresponding DIC images. Insets show the area indicated by orange dashed boxes, magnified 2-fold; they illustrate the efficient diffusion of
cwn-2p::CWN-2::Venus but not of
cwn-1p::CWN-1::Venus. (C) Expression of
cwn-2p::CWN-2::Venus in the pharynx and near the intestine. Orange, white, and blue lines outline the pharynx, intestine, and gonad, respectively. Puncta of
cwn-2p::CWN-2::Venus near the intestine (white arrowheads) were observed only in the anterior region. Puncta in the intestine are gut granule autofluorescence. (D, E) Diffusion of
ceh-22p::CWN-2::Venus (E) but not
ceh-22p::CWN-1::Venus (D) was observed in the anterior hypodermis near the pharynx. White arrow heads, blue arrows and the orange arrow indicate GFP puncta, hypodermal cells expressing the
elt-3::GFP marker, and neuronal processes expressing the
mec-4::GFP marker, respectively. (F-H) Each colored box represents the polarity of individual seam cell divisions, as in Figure 1C. (F) The
egl-20 phenotype was not affected by EGL-20 expression in the M cell (
hlh-8p::EGL-20). Since the M cell is on the right side of the animals, we scored seam cells only on the right side. (G)
cwn-1p::CWN-1::Venus rescued the defect of the
cwn-1 mutation in
cwn-1;
egl-20. (H) The phenotype of
cwn-1;
egl-20 cwn-2 was rescued by
cwn-2p::CWN-2::Venus, by CWN-2 expressed in the pharynx (
ceh-22p::CWN-2::Venus) or in the cells near the anus (
egl-20p::CWN-2::Venus#1 and #2 with
mec-4::GFP and
egl-5::GFP as coinjection markers, respectively), or by CWN-1 expressed in the pharynx (
ceh-22p::CWN-1::Venus).