Figure 3. Localization of PCP Components, SAX-3/ROBO, and NMY-2 during Rosette Assembly(A-C) Neuronal but not epidermal-specific expression of (A)
vang-1, (B)
prkl-1, and (C)
sax-3 rescue the anterior displacement of DD neurons in the correspondingmutant backgrounds. DD neuron quantification and color scheme is as described in Figure 2F. Each DD2-6 neuron in transgenic worms (TG+) are compared withtheir corresponding neuron in non-transgenic (TG) siblings. Means and 95% confidence intervals are shown for each DD neuron. **p % 0.001, using the twotailed t test (n = 44-62 worms).(D-G) Partial maximum projections of volumes acquired of embryos expressing the
cnd-1 membrane label and functional GFP fusions with (D) PRKL-1 (zyIs33),(E) VANG-1 (
zy60), (F) NMY-2 (
cp13), and (G) SAX-3 (zyIs43) during rosette formation. Dashed ovals in early time point mark a contracting cell contact; dashedcircles at later time point mark the resulting rosette vertex.(H) Enrichment of VANG-1::GFP and NMY-2::GFP at the contracting cell-cell contact during rosette assembly relative to mean GFP levels at non-contracting cellcontacts (n = 3).(I) Enrichment of VANG-1::GFP and NMY-2::GFP at the rosette center after rosette formation relative to mean GFP levels at the pairwise cell contacts between allcells in the rosette (n = 3). Error bars show SE.Scale bars, 5 um (D) and 2 um (E-G).