Figure 6. MIG-14 Trafficking and TransportRequire AP2, Retromers, Kinesin 1, and Dynein(A) Z stack confocal projection images of MIG-14distribution in PVD of the wild-type,
dpy-23, or
vps-35mutants revealed by a MIG-14::mCherry transgene.The PVD was labeled by wdIs52(F49H12.4::GFP),which is integrated on the X chromosome. wdIs52could not be recombined with the
dpy-23(
e840) allele,which is also on X. Therefore, no dendrite channel wasshown for
dpy-23. Boxed regions are highlighted onthe right, with mCherry (MIG-14, pseudocolored inwhite) and GFP (PVD, pseudocolored in blue)/mCherry merged images shown. Arrowheads indicatetertiary dendrites. The scale bar represents 10 um.(B) Quantification of diffuse MIG-14::mCherry signal inthe dendrites. Error bars are SEM. N equals numbersof neurons scored. *p < 0.05; ***p < 0.001; one-wayANOVA followed by Bonferroni's multiple comparisontest.(C) Quantification of dendrite self-avoidance defectsin the wild-type,
dpy-23, or
vps-35 mutants. Errorbars are SEM. N equals numbers of neurons scored.*p < 0.05; **p < 0.01; one-way ANOVA followed byBonferroni's multiple comparison test.(D) Kymographs (top) and quantification (bottom) ofMIG-14::mCherry movement in the primary dendritesof the wild-type,
unc-116, and
dhc-1 mutants. (Top)Arrows indicate stationary MIG-14 signals. (Bottom) Nequals numbers of tracks analyzed. ***p < 0.001; twoproportion z test with Bonferroni correction.(E and F) Z stack confocal projection images (E) andquantification (F) of diffuse dendritic MIG-14::mCherrysignals in the wild-type,
unc-116, and
dhc-1 mutants.(E) Arrows and colors are as in (A). The scale barrepresents 10 um. (F) Quantification of diffuse MIG14::mCherry signal in the dendrites. Error bars areSEM. N equals numbers of trajectories scored. *p <0.05; **p < 0.01; one-way ANOVA followed byBonferroni's multiple comparison test.(G) Dendrite self-avoidance defects in the wild-type,
unc-116, and
dhc-1 mutants. N equals numbers ofanimals scored. Error bars are SEM; one-way ANOVAfollowed by Bonferroni's multiple comparison test.