dve-1 Encodes a Nuclear Protein Essential for Maintenance of Mitochondrial Mass and Morphology(A) Fluorescent photomicrographs of wild-type and
dve-1(RNAi) embryos immunostained with antiserum to DVE-1 (left panels) and a similarly staged wild-type embryo bearing a
dve-1pr::
dve-1::gfp transgene (right panel). The embryos were counterstained with the DNA-binding dye H33258, revealing the nuclear morphology (lower panels).(B) A time-lapsed series of images of a
dve-1/+ embryo (lower right) and a sibling
dve-1(
tm259)X mutant embryo (upper left) photographed under phase contrast microscopy. (C) Fluorescent photomicrographs of wild-type (N2) and
dve-1(
tm259)X mutant embryos transgenic for the UPRmtreporter
hsp-60pr::gfp (SJ4203). Where indicated, the animals were subjected tospg-7(RNAi). (D) Fluorescent photomicrographs of wild-type and
dve-1(
tm259)X mutant embryos immunostained for DVE-1 and costained with Mito-Tracker and H33258. (E) Fluorescent photomicrographs of the DVE-1 immunostain, AvidinFITC stain, and H33258 labeling of sibling wild-type (
dve-1/+,on right) and mutant embryos (
tm259, left). (F) Fluorescent photomicrographs of body wall muscles of animals transgenic for a mitochondrially localized GFP reporter (
myo-3pr::GFPmt). Where indicated, the animals were subjected to inactiva-tion of
spg-7, known to induce mitochondrial unfolded protein stress,
ire-1, a negative control that induces ER stress and
dve-1.