Figure 1. C. elegans DOS-Motif Proteins Are Required for Response to Octanol (A) Octanol responses of animals carrying the likely null alleles osm- 11
(rt142),
osm-7(
tm2256),
dos-1(
ok2398), and partial loss-of-function allele
dos-2(
tm4515). p < 0.0005 versus wild-type (WT). (B)
osm-11 function is not required during development for octanol response in adults. $p < 1024 versus control (RNAi); p > 0.05 versus WT; #p > 0.05 versus
osm-11(null);
osm-11(OE) no heat shock (HS). (C) OSM-11 protein accumulates in coelomocytes.
osm-11p::gfp reporter constructs do not drive GFP expression in coelomocytes [4]. Top left: representative image of OSM-11 accumulation in a coelomocyte using aOSM-11 antiserum [4]. Bottom left: osmotic stress likely results in diminished OSM-11 secretion by seam cells into pseudocoelom. Top and bottom right: GFP expression (
unc-122p::gfp) in coelomocytes [53]. Scale bar represents 6 mm. Time to respond to octanol in (A) and (B) is reported as mean time to initiate backward locomotion (s) 6 standard error of the mean (SEM).