Figure 6. Intestinal lineage organization, but not specification, is disrupted in
emb-4 embryos.
emb-4(
qm31) males were crossed into transgenic animals expressing the E-lineage (intestinal)-specific GFP marker
elt-7 (green, bottom row). Both
elt-7::gfp and
elt-7::gfp;
mal-2(
qm31) transgenic animals were dissected and stained with DAPI (red/middle row). While late-stage
elt-7::gfp;
emb-4(
qm31) embryos arrest as masses of poorly differentiated embryos (C, right column), the number of GFP-expressing cells at all stages (A-C, right column) is identical to wild-type animals expressing
elt-7::gfp (A-C, left column and data not shown).