Figure 1. Expression pattern of
etr-1. GFP fluorescence pattern of animals carrying a
rol-6 marker and a non-integrated extrachromosomal array of a GFP-lacZ reporter (derived from plasmid pD96.04, a gift from A. Fire) fused to promoter elements of
etr-1. In early embryos, muscle cells are initially seen as a continuous sheet on the lateral side of the embryo. Between 300 and 350 min, starting from the anterior, the sheet begins to separate as the cells move to form two dorsal and two ventral muscle quadrants. (a,b) Dorsolateral view of GFP expression in an approximately 300 min embryo is seen in a muscle sheet which is just beginning to separate at the anterior. (c,d) 1.5-fold-stage embryos (approximately 430 min) express GFP in the four muscle quadrants along the length of the embryo (two of which are seen clearly in this example). (e) Adult animals show expression in body-wall muscle, seen here in stripes along the length of the adult hermaphrodite. The four muscle quadrants spiral along the length of the animal because of the presence of the
rol-6 marker. Anterior is to the left. (f,g) Ventral view of vulval muscle. (h,i) Intestinal muscle seen in lateral view with the ventral side to the left. (j,k) Lateral view of anal sphincter muscle (arrowhead) with dorsal at the top. (b,d,g,i,k) The corresponding Nomarski images of the fluorescence images shown in (a,c,f,h,j).