S1 Fig. The C. elegans specific
era-1 gene contributes to robust embryonic development.A. Schematic of the wild-type
era-1 gene and of
era-1(
tm5852) and
era-1(
tm6426) mutant alleles, with an indication of premature STOP codons (lack of translation is depicted in grey). Analysis with Scanprosit [1] failed to identify clear protein domains in ERA-1. B. Phylogenetic relationships of ERA-1-related proteins amongst selectnematodes identified by Blast search (Ensembl). The tree was built with the Clustal W2 software
(http://www.ebi.ac.uk/Tools/phylogeny/clustalw2_phylogeny/) on sequence alignment performed with the MAFFT server
(http://mafft.cbrc.jp/alignment/server/). The values indicate the length of the branch leading to the previous node and show thenumber of substitutions as a proportion of the alignment length. C. Embryonic lethality following partial depletion of PAR-3 or PAR-2 in wild type and
era-1(
tm6426) worms. >800 embryos were scored in 8 independent experiments for
par-3(RNAi), >200 embryos in 3 independent experiments for
par-2(RNAi). Note that no enhancement was observed upon
glp-1(RNAi) and
apx-1(RNAi) as compared to wild type, indicating that the enhanced lethality observed with
par-3(RNAi) and
par-2(RNAi) is not a generic effectin response to RNAi. Statistical analysis was performed using unpaired Student's t-test tocompare embryonic lethality in wild type versus
era-1(
tm6426) in the indicatedconditions, yielding the following p-values: partial
par-3(RNAi), p = 4.610-3; partialpar-2(RNAi), p = 0.048. D-E. YFP-ERA-1[3'
era-1] protein at telophase of the first celldivision (D) and in the 2-cell stage (E). The upper images show the YFP signal alone, thelower ones the merge. Scale bar represents 10 microns.1. de Castro E, Sigrist CJ, Gattiker A, Bulliard V, Langendijk-Genevaux PS, Gasteiger E,et al. (2006) ScanProsite: detection of PROSITE signature matches and ProRuleassociatedfunctional and structural residues in proteins. Nucleic acids research 34:W362-365