Supplemental Fig. S2 RNAi efficiency of
cytb-5.1 and
cytb-5.2.(A-C) Relative mRNA expression of
cytb-5.1 and
cytb-5.2 treated by
cytb-5.1RNAi orcytb-5.2 RNAi, respectively. The percentage (%) reduction of
cytb-5.1 and
cytb-5.2expression by RNAi was indicated.(D) The fluorescence of CYTB-5.1::GFP and CYTB-5.2::GFP treated with
cytb-5.1RNAi orcytb-5.2 RNAi, respectively. The scale bar represents 200 um.(E) Relative fluorescence intensity of CYTB-5.1::GFP and CYTB-5.2::GFP from (D) (n=3).Data are presented as the mean +- SD of at least three independent replicates. Significantdifferences between control (EV) and
cytb-5.1RNAi worms or
cytb-5.2RNAi worms: *: P <0.05, **: P < 0.01, and ***: P < 0.001.