Figure 6. GFP reporters validate NSM-enriched RNA-Seq data set. Transgenic animals expressing promoter::GFP reporter genes for NSMenriched transcripts. Confocal images of adult hermaphrodites, anterior to left, ventral down. A-B, Bright
tph-1::GFP (vsIs45) expression is limited to NSML and NSMR; note dim
tph-1::GFP signal in ADF neurons (arrows). C, Cartoon depicting NSM neurons marked with
tph-1::GFP in the anterior pharyngeal bulb. Y39B6A.19::GFP (D, E) and F41E7.3::GFP (F, G) are specifically expressed in neurons, including NSM, head neurons and ventral cord motor neurons (asterisks). Y39B6A.19::GFP is also expressed in sensory neuron, PDE (E). Scale bar is 25 microns.