Figure 2. Genes Expressed in GABAergic Motor Neurons. (A) Expression patterns of group A promoter-GFP reporter constructs in L1 larval animals.Orientation as in Figure 1A. This group of genes showed expression in the D-type GABAergic neurons as well as other neurons.The commissural pattern illustrated by arrowheads is diagnostic for DD neurons in L1 larvae(upper left). The ventral cord is indicated by an arrow in all panels. Arrowheads mark GFP-expressing neuronal cell bodies where some of them are the D-type neurons (bottom panels). Reporter constructs are based on the promoter sequences of F53B6.2a, a conserved member of immunoglobulin superfamily;
acr-12, an acetylcholine receptor subunit;F36A2.4/twk-30, potassium channel protein; and
rpy-1, rapsyn. Bar, 10 m.(B) Expression of group B genes and their regulation by UNC-30. Expression patterns of promoter-GFP reporter strains in L1 larval wild-type (left panels) and
unc-30 mutant backgrounds (right panels) were shown forunc-25 [7],
acr-14, C55F2.2,
flp-13, and
oig-1. Orientation as in Figure 1A. Bar, 10 um.