Figure S3. MYRF-1 exhibits cytoplasmic and nuclear localization.Related to Figure 3.A. Illustration of knock-in allele (
ybq14) of
myrf-1(F59B10.1), in which GFP is inserted after Ala171 and 3xFlag isinserted at the C-terminus.B. Immunostaining on GFP::
myrf-1 knock-in (
ybq14) animals using anti-GFP. DAPI, a DNA-binding dye. Left panelsshow head region, and right panels, trunk region.C. Protein extracts from GFP::
myrf-1 knock-in (
ybq14) and N2 animals are analyzed by Western Blot. Two bands (140kDa, 75 kDa) are detected by anti-GFP.D. Immunostaining on Flag tag inserted in full length MYRF-1, N-MYRF-1(1-482aa), or C-MYRF-1(483-931aa),expressed in HEK293 cells by transfection. Co-expressed is ER::DsRed or Golgi::DsRed. DAPI, a DNA binding dye.Scale, 5 um.