Figure 4. Subcellular expression of GRO-1::GFP. (A-D) GFP fluorescence in a muscle cell in three different planes of focus to show how the fluorescence pervades the entire cytoplasm and nucleus. The weak striations that can be observed are the results of partial exclusion of the fluorescence from the contractile elements of the muscle. The dark granules that can be seen in D are not mitochondria but some other unidentified cytoplasmic inclusions. (E) Mitochondrial localization of the long form of GRO-1 [product of
gro-1(Met1- 5Ile)::gfp; see main text]. The mitochondrial distribution of CLK-1 is shown in H for comparison. (F) A muscle cell adjacent to the vulva expressing the short form of GRO-1 [product of
gro-1(Met1Ile)::gfp; see main text]. Only one plane of focus is shown. The observed distribution is indistinguishable from the distribution of GRO-1::GFP as shown in A-D. (G) Distribution of GRO-1::GFP in a neuron. Note the fluorescence is also found in the neuronal projection and that it is slightly less intense in the nucleus. (H) The mitochondrial distribution of CLK-1 (Felkai et al. 1999). (I) Distribution of the long form of GRO-1 in the mitochondria of a neuron. Note the granular cytoplasmic distribution and the exclusion from the nucleus.