Figure 3. Morphological characterization of large vacuoles in coelomocytes of
cup-5(
ar465) worms. Shown are confocal images of WT or of
cup-5(
ar465) coelomocytes expressing the indicated GFP fusions (green) 24 h after BSA-Rhod (red) injection into the body cavity. Large arrows indicate large vacuoles, small arrows indicate lysosomes, arrowheads indicate late endosomes, the asterisk indicates heterogeneity in BSA-Rhod staining within the large vacuoles, and the small compartments stained by mannosidase II::GFP (MAN::GFP) are indicated with a G. Coelomocytes are outlined. (Bar, 5 um in all images.)