S2 Fig. Dynein targeting to MT tips depends on dynactin.(A) Cortical confocal section of one-cell embryos in metaphase co-expressingendogenous dynein heavy chainDHC-1::GFP and EBP-2::mKate2, showing that depletionof
p150DNC-1delocalizes dynein from MT tips. Images are maximum intensity projectionsover time (12 images acquired every 5 s). Scale bar, 5 um; insets, 2 um.(B) Quantification of dynein heavy chainDHC-1::GFP levels at MT plus ends usingfluorescence intensity measurements at the cortex. Error bars represent the SEM with
a95% confidence interval, and n indicates the total number of measurements from 7-8embryos per condition. The t-test was used to determine statistical significance. ****P <0.0001.(C) Central confocal section of metaphase one-cell embryos expressing transgeneencoded mKate2::EBP-1, demonstrating the efficiency of
ebp-1/3(RNAi). Images aremaximum intensity projections over time (10 images acquired every 300 ms). Note thatfor reasons that are not clear, mKate2::EBP-1 does not localize to MT plus ends. Scalebar, 5 um.(D) Schematic of the
clip-1 locus. Mutations introduced by CRISPR-Cas9-based genomeediting to generate a null allele (
clip-1) are indicated in black font.