Figure 1. Expression pattern of
lin-3 and
let-23 reporter constructs in hermaphrodites during development of the uterine-vulval connection. (a) Schematic representation of cell positions in the early phase of the developing uterine-vulval connection. (b,c) GFP and Nomarski lateral images of a
let-23::GFP transgenic hermaphrodite in the mid-L4 stage showing
let-23::GFP expression in the
uv1 cells (arrows) as a thin lateral ring connecting the uterus to the vulva. The
let-23::GFP construct was expressed from the
let-23 promoter in the syEx234 transgenic array in a wild-type animal. (d) Ventral view of a mid-L4 larva showing expression of beta-galactosidase from the
lin-3::lacZ reporter construct in the vulF descendants of P6.p (arrows). (e) Ventral-lateral view of a mid-L4 larva showing
lin-3::lacZ expression in the vulF cells (arrows). (f) Lateral view of a mid-L4 larva with a multivulval phenotype showing
lin-3::lacZ expression in all the vulval invaginations (arrows).