Figure 6. GFP::LIT-1 Nuclear Asymmetry between A-P Sisters Is Reciprocal to GFP::POP-1 Nuclear Asymmetry. Micrographs showing GFP::LIT-1 fluorescence (A, C, E, and G) and DIC images (B, D, F, and H) in wild-type or
mom-2(RNAi) backgrounds. (A, B, G, and H) eight-cell, (C and D) 12-cell, (E and F) 14-cell. Images are selected from movie recordings. The black line in each panel connects a pair of A-P sister nuclei whose names are indicated and which are equally focused at that particular focal plane. (I) Relative levels (A/P%) of nuclear GFP::LIT-1 between MS and E (left-hand graphs) and ABxxa and ABxxp (right-hand graphs) in wild-type,
mom-2(RNAi),
wrm-1(RNAi),
mom-5(RNAi), and
mom-4(RNAi) embryos. *p < 0.001. Scale bar, 10 μm.