- Picture from Karabinos A et al. (2003) J Mol Biol "In vivo and in vitro evidence that the four essential intermediate filament (IF) ...."
Figure 4. Colocalization of B1 and MH4 antigens in C. elegans embryo and larva. The left half of the Figure gives rabbit anti-B1 specific immunofluorescence of embryos (A, C and E) and the larva (G) that were double-stained with the murine monoclonal antibody MH4 that detects proteins A1 to A3 (right half of the Figure). The arrowheads highlight coexpression/co-organization of the B1 and the MH4 antigens in the dorsal and ventral hypodermis (except for the seam) of the comma (A and B), 1.5-fold (C and D) and 3-fold (E and F) stage embryos and the larva (G and H).
- Picture from Edens WA et al. (2001) J Cell Biol "Tyrosine cross-linking of extracellular matrix is catalyzed by Duox, a ...."
Figure 4. Cellular expression of Ce- Duox1. Animals were immunostained with antibodies to Ce-Duox1 (A-C, E, F, and H, green), myosin A (A and B, red), and MH4 (D, E, G, and H, red), and fluo- rescence was visualized using confocal microscopy. Merged images of Ce-Duox1 and myosin A staining (A and B) show that the Ce-Duox1 is expressed in a layer of cells just outside the muscle bundles. Longitudinal images (C and D) and cross section images (F and G) show the indi- vidual staining patters of Ce-Duox1 and the hypodermal cell marker MH4, respectively. Merged images (E and H) show areas of overlapping staining of Ce-Duox1 and MH4 in yellow, confirm- ing the expression of Ce-Duox1 in the hy- podermal layer of cells. The use of antibody to Ce-Duox1 that had been preincubated with Ce-Duox1(340-355) peptide eliminated green channel anti- body staining (unpublished data). Photos are representative of >100 animals observed.
- Picture from Ding M et al. (2003) Development "C. elegans ankyrin repeat protein VAB-19 is a component of epidermal attachment ...."
Figure 4. VAB-19 localizes to epidermal attachment structures. (A-I) VAB-19::GFP progressively co-localizes with intermediate filaments. Confocal images of VAB-19::GFP expression (juIs167) visualized using anti-GFP immunostaining (green); intermediate filaments were visualized using the MH4 monoclonal antibody (red). At the comma stage (A-C), VAB-19::GFP partly colocalized with intermediate filaments. At the intermediate elongation stage (D-F), VAB-19::GFP and intermediate filaments both localized to muscle-adjacent regions of the dorsal and ventral epidermis. During later embryogenesis and larval stages until adulthood, staining of anti-GFP and MH4 was coincident. (G-I) L1 stage epidermis in z-axis section. (J-L) Lateral view of adult epidermis. (M-R) Colocalization of VAB-19::GFP and Myotactin (MH46 antigen). At the intermediate elongation stage (M-O), VAB-19::GFP and Myotactin are both localized to epidermal cell regions adjacent to muscle. In adults (P-R), the bands of Myotactin and VAB-19::GFP are interrupted by gaps corresponding to the positions of neuronal processes (arrows), at which positions MH4 staining is usually enhanced (K, arrow). Like Myotactin, but unlike MH4 staining, VAB-19::GFP is absent from such process gaps (compare J and P). VAB-19::GFP was also expressed in pharyngeal marginal cells (S); unlike Myotactin (T), VAB-19::GFP was localized throughout the apical basal axis of these cells and was more concentrated at the apical and basal surfaces. Scale bars: 5 um (G-I); 10 um (all others).
- Picture from Karabinos A et al. (2001) Proc Natl Acad Sci U S A "Essential roles for four cytoplasmic intermediate filament proteins in ...."
Figure 4. Expression of A1-, A3-, and B1-promoter/gfp constructs and the IF protein B1. Expression of the A1 promoter/gfp reporter in cells associated with amphid sensory neurons (A), the pharyngeal-intestinal valve (C), the rectum, and some neurons of the tail (E). Similar structures were immunostained with antibody MH4 which, however, also decorates the hypodermis (B, D, F, and J). The A3 promoter/gfp reporter is primarily expressed in the embryonal and larval hypodermis (G). The B1 promoter/gfp is expressed in the rectum and some unidentified neurons in mixed-stage animals (H). Whole-mount worms were double-labeled with affinity-purified rabbit antibody specific for IF protein B1 (I) and with antibody MH4 (J). [Scale bars: 50 um (A, C, E, and H) and 25 um (B, D, F, G, I, and J).]
- Picture from Hong L et al. (2001) J Cell Biol "MUP-4 is a novel transmembrane protein with functions in epithelial cell ...."
Figure 7. MUP-4 expression in larvae. (A) Lateral view of an N2 larva (L2) stained with a MUP-4 antibody. Arrows mark staining of MUP-4 in circumferential rings, arrowheads mark B cell perinuclear staining. (B) Lateral view of an L2 larvae MUP-4::GFP expression in an integrated mup- 4::gfp line (EE82). Arrows mark staining in circumferential rings, arrowheads mark B cell perinuclear staining. Small arrow marks diffuse hypodermal cell staining in hypodermal cells overlying muscle. (C) Head of an L1 larva showing MUP-4::GFP fluorescence (EE73; arrow). (D) Confocal overlay of immunolocalization of MUP-4 (red) and MH27 and MHCA (green). MH27 demarcates seam cell boundary (arrowhead). (E-G) Confocal analysis of an L3 larva stained with MUP-4 and MH4 showing localization to circumferential rings (arrows) and the touch neuron (arrowheads). The confocal overlay (G) shows partial colocalization.
- Picture from Bosher JM et al. (2003) J Cell Biol "The Caenorhabditis elegans vab-10 spectraplakin isoforms protect the ...."
Figure 5. VAB-10A and VAB-10B form alternating circumferential bands in the larval epidermis. (Top) Confocal projections (A, B, C, D, and E-E''), and optical section through the apico-basal axis along the area marked with a double arrow (A', B', C', and D') after staining wild-type adult animals (A/A', D/D', and E'/E'') or L1 larvae (B/B' and C/C'); mAb staining is in red. (A/A') VAB-10A pAbs and myosin heavy chain-specific mAb 5.6.1.1; note the respective orientations (A) and thickness (A') of sarcomeres and FOs. (B/B') VAB-10A pAbs (green) and mAb MH4 (IFs; red); both proteins colocalize. (C/C') VAB-10A pAbs and mAb MH46 (myotactin); these proteins do not generally colocalize. (D/D') mAb MH5 (VAB-10A; green) and VAB-10B K22 pAbs (violet); these proteins do not colocalize and VAB-10B extends slightly further than VAB-10A. (E-E'') Differential interference contrast picture (E) of the animal immunostained with VAB-10B K22 pAbs (E'); the merged image (E'') shows that VAB-10B is found at the furrows separating annuli (arrowheads; due to the permeabilization treatments, their morphology is rather poor). The K32 antiserum revealed the same pattern, but it was much fainter (not depicted). Bar, 10 um (A and D), 2.5 um (B and C), or 5 um (E). (Bottom) Immunogold- labeled micrographs showing the positions of VAB-10A (F and G), and VAB-10B (H) obtained with 4F2 and K22 antibodies, respectively. In the epidermis, VAB-10A (but not VAB-10B) is enriched in FOs (arrowheads, gold particles; white arrows, dense bodies; F and G are from different sections). The specificity of staining is indicated by the signal-to-noise ratio measured by counting gold beads in sections stained with 4F2 or K22 primary antibodies, versus in control sections without primary antibody. 4F2: epidermis, 36.7 ± 4.6, and muscle, 0.9 ± 0.2; K22: epidermis, 4.3 ± 0.5, and muscle, 3.7 ± 0.6 (n = 2). Bars: 500 nm (F and H) and 100 nm (G).