Figure 2. EIF-3.K protein expression. (A) Western blot analysis of EIF-3.K protein expression. Affinity-purified anti-EIF-3.K antibodies were used to probe a blot of embryonic extracts from wild-type and
eif-3.K
(gk126) worms (above). Equal loading of the two extracts was confirmed by anti-a tubulin antibodies (below). The sizes of molecular weight markers and the positions of EIF-3.K and a tubulin are indicated. (B-D) Images of an
eif-3.K
(gk126)mutant early embryo (B), a wild-type early embryo (C) and a wild-type newly hatched larva (D) that were co-stained with anti-EIF-3.K antibodies and DAPI. Merged images are also shown. Scale bar = 10 um.