Figure 1. RNAi knockdown of
dcaf-13 impairs C elegans growth, fertility, and development.:A-C) Representative images of 2-day old A) L4440 control B) Ahringer library
dcaf-13(RNAi) or C) Vidal library
dcaf-13(RNAi) treated C. elegans. Images were taken at 25X magnification, scale bar=0.25mm. D) Quantification of worm lengths for 2-day old L4440 control or
dcaf-13 RNAi treated C. elegans. Graph shows combined data from 3 independent biological replicates, n=10 for each replicate. Treatment with both Ahringer (AR) and Vidal library
dcaf-13 RNAi clones resulted in a significant reduction in worm length compared to L4440 control (One-way ANOVA with Sidak's multiple comparisons, p<0.001). E) Total progeny produced by 3 adult worms during a 12-hour laying period. L4 stage worms were treated with control or
dcaf-13 RNAi for 24 hours, then allowed to lay eggs for 12 hours before being removed. 24 hours after adult removal, embryos and larvae were counted. Treatment with both AR and Vidal
dcaf-13 RNAi resulted in less progeny production compared to L4440 control treatment (One-way ANOVA with Sidak's multiple comparisons, n=4, p<0.05). Data shown is from four independent biological replicates. F) Percent embryonic lethality was calculated by dividing the number of unhatched embryos by the total number of unhatched embryos and live larvae produced by RNAi or control treated worms at least 24 hours after egg laying and adult removal. No statistically significant differences in percent embryonic lethality were found (Kruskal-Wallis test, n=4, p=0.08). Data shown is from four independent biological replicates. G) Larvae produced during a 12-hour egg laying window by
dcaf-13 RNAi or control treated C. elegans were followed for 72 hours after egg laying and the number of larvae to have reached L4 stage at each time point was recorded. None of the
dcaf-13(RNAi) larvae had reached L4 stage as of 72 hours post egg laying. Data shown is the average of 2 independent biological replicates. *p<0.05, ****p<0.001