FIGURE 3. Gene expression and orthologs of K10C3.4. (a) Coding regions and UTRs are drawn in green and red, respectively. The structures of plasmid constructs used in this study are also shown. (b-d) The expression of a GFP reporter containing the entire promoter (b), upstream promoter (c), and downstream promoter (d). (e,f) The localization of a GFP fusion protein in vulval cells (e) and in seam cells (f) of the same animal. All worms are at the L4 stage and the anterior is to the left. (g) Amino acid identities between orthologs. (Ce) C. elegans; (Cb) C. briggsae; (Cr) C. remanei; (Hs) Homo sapiens Transmembrane protein 98. Transmembrane domains are shown in black. (h) Alignment of 39 UTR sequences. Numbers represent the leftmost nucleotides in base pairs downstream of the start of the 39 UTRs. Conserved sequences complementary to the
let-7 seed are surrounded by large rectangles. A nonconserved complementary sequence is denoted by a small hashed rectangle. Nucleotide substitutions are shown on the sequences along with the site number. An arrow indicates the 39 terminus of the cloned sequence.