Figure 2.-OCR-2(Y395F) is an unstable dominant-negative subunit whose effects are suppressed by wild-type OCR-2. (A and B) Expression of OCR-2TGFP and OCR-2(Y395F)TGFP. Fluorescence images showing expression of full-length OCR- 2TGFP (A) and full-length OCR-2(Y395F)TGFP (B) in cell bodies (brackets) and cilia (arrowheads) of sensory neurons in the tail. OCR-2(Y395F)TGFP is an unstable protein. Bars, 30 μm. (C) OCR-2(Y395F) dominantly causes premature egg-laying behavior. Wild-type OCR-2 or OCR-2(Y395F) was transgenically overexpressed under the control of the
ocr-2 promoter and the 39 regulatory region in a wild-type back- ground. (D) Effect of varying dosage of
ocr-2 alleles on egg laying. Animals carrying various combinations of
ocr-2 alleles were assayed for premature egg laying. 1,
ak47, and
vs29 de- note wild-type, null, and
vs29 alleles of
ocr-2, respectively. Brackets with asterisks indicate significant differences (P , 0.05). Each strain analyzed was heterozygous for the marker mutation
dpy-20(1282ts), which was necessary to verify some genotypes and was included in all others for consistency.