Figure 4.
paqr-2 is essential for growth at 15 C. (A) The fraction of individual L1 larvae that grew into fertile adults is shown for different cultivation temperatures. The grouped genotypes are the same as for Fig. 1. (B) and (C) Show wild type or
paqr-2 mutant worms grown for 120 hours at 15uC, while (D) shows the growth rescue of
paqr-2 worms carrying the pPAQR-2::N-GFP as a transgene. The pPAQR-2::GFP-C transgenics show no visible green fluorescence (E) while the pPAQR-2::N-GFP transgenics show expression of GFP on the plasma membranes of head and tail neurons as indicated by arrows in (F), with (G) being en enlarged view of the area boxed in (F) where the arrowhead indicates a bright neuron that is slightly out of focus. (H) Shows the average length of wild type worms,
paqr-2 mutant worms, and
paqr-2 mutants transgenic lines carrying the pPAQR-2 transgene (2 lines, L1 and L2), the pPAQR-2::GFP-C transgene (2 lines, L1 and L2) and the pPAQR-2::N-GFP transgene (1 line). *p,0.05, ** p,0.01 and *** p,0.001 using the z-test in (A) and the unpaired Student's t-test in (H). Note that in (A)
paqr-2 is also significantly different from controls at 20uC and 25uC with p,0.01 and p,0.05, respectively. Scales indicate 500 mm (B-D), 100 mm (E-F) and 20 mm (G); error bars show the sem.