Figure 5. APR-1 expression pattern during larval development. (A) APR-1::GFP expression and (B) mAb MH27 staining of an L1 larva carrying the zhIs2[
apr-1::GFP] array. The vertical arrows in A and B point at the six pairs of P cells that were descending from the left and right sides toward the ventral midline, the horizontal arrow points at the excretory cell body. (C) APR-1 antibody and (D) mAb MH27 staining of an L3 larva carrying the zhEx2[
apr-1wt]array. Note the APR-1 expression in the VPCs (P4-8.p) indicated by the vertical arrows and in the seam cells indicated by the arrowheads. In this animal, P3.p had adopted an F fate (as observed in 39% of wild-type animals) and did not express APR-1. No APR-1 expression was detectable in the anterior (P1-2.p) or posterior (P9-11.p) Pn.p cells (P1.p, P10.p, and P11.p are not shown). (E) Higher magnification of APR-1 antibody, and (F) mAb MH27 staining of P3-5.p in an animal in which P3.p had adopted a VPC fate. All larvae are shown from a lateral view, anterior is left. Scale bars inD and F, 10 μm.