Figure 4. PGL-1 Is a Constitutive Component of P Granules.Immunofluorescence micrographs of embryos, larvae, and extruded adult hermaphrodite gonads. Anterior is left; ventral is down.(A-D) Wild-type late 1-cell embryo costained with rabbit anti-PGL-1 antibody (A), mouse monoclonal antibody K76 (B), and the DNA dye, DAPI (D). The merged image (C) demonstrates double staining of granules by anti-PGL-1 and the original anti-P-granule monoclonal antibody, K76.(E-H) Wild-type early embryos stained with anti-PGL-1. P granules in the germline blastomere, P1 (E), P2 (F), P3 (G), and P4 (H) of a 2-, 4-, 8-, and 24-cell embryo, respectively, are stained.(I and J) Wild-type L1 (I) and L2 (J) stained with anti-PGL-1. P granules surround the germ nuclei.(K and L) Wild-type adult hermaphrodite gonad stained with anti-PGL-1 (K) and DAPI (L). P granules are perinuclear in the distal arm (upper left) and dissociate from the nuclear membrane during oogenesis (lower right).(M and N)
pgl-1(
bn101) adult hermaphrodite gonad costained with rabbit anti-PGL-1 (M) and mouse anti-GLH-1 (N). P granules fail to stain with anti-PGL-1 but stain with anti-GLH-1.(O-Q)
glh-1 adult hermaphrodite gonad (O) and early embryos (P and Q) stained with anti-PGL-1. PGL-1 is present but not associated with P granules. Bars, 10 μm.